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Leptospira interrogans (Stimson) Wenyon

23476™

Reference strain for serotype Autumnalis.

Product category

Bacteria

Strain designation

Akiyami A

Type strain

No

Geographical isolation

Japan

Product format

Frozen

Storage conditions

-80°C or colder

General

Preceptrol

No

Characteristics

Serotype

Autumnalis

Comments

Reference strain for serotype autumnalis

Handling information

Medium

ATCC Medium 1470: Modified Leptospira medium

Temperature

30°C

Atmosphere

Aerobic

Handling procedure

TRANSFER IMMEDIATELY upon thawing by aseptically withdrawing cell suspension from vial and inoculating a 10-12 mL tube of Medium #1470, inserting the pipette just below the surface of the semi-solid medium.  Aliquots of 0.5 ml from this tube may be used to inoculate additional tubes if needed.  An aerobic blood plate may also be streaked to test for purity.

Incubate test tubes with screw caps lightly tightened along with the blood plate at 30°C under aerobic conditions.

Initial growth may take up to 7 to 14 days.  Observe growth as a thin, tight band forming just below the surface of the medium.  The band thickens as incubation continues.  Cellular morphology under phase contrast shows motile flexible helical rods in singles.

The aerobic blood plate should show no signs of growth.

Handling notes

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

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