平台编号：Bio-134992

Anabaena flos-aquae (Lyngbye) Brebisson

22664™

Product category

Bacteria

Type strain

No

Geographical isolation

United States; Wisconsin

Product format

Freeze-dried

Storage conditions

2°C to 8°C

General

Specific applications

Toxicity testing

Fixes nitrogen

This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E1218-90 where only the taxon is specified.

Preceptrol

No

Characteristics

Comments

Non-axenic

Handling information

Medium

ATCC Medium 625: Gorham's medium for algae

Temperature

25°C

Incubation

Under light intensity of 150 foot candles

Handling procedure

Open vial according to enclosed instructions or visit www.atcc.org for instructions.

Rehydrate the entire pellet with approximately 0.5 mL of #625 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #625 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.

Use several drops of the primary broth tube to inoculate a #625 agar slant.

Incubate at 25°C under light intensity of 150 foot candles. It is helpful to incubate test tubes in a slanted position to increase gas exchange in broth and to enhance exposure to light.

Handling notes

This item is non-axenic

Good growth, indicated by increased pigmentation in the broth or on the slant, should occur after one to two weeks of incubation.  Examine cells microscopically to assure that they are intact and healthy.  At this time additional test tubes or flasks can be inoculated.  A 5% inoculum is recommended (i.e. 5 mL of culture to 100 mL fresh medium).

To minimize change in a culture, it is recommended that a frozen seed stock be established from early passage cells.  This may be accomplished by propagating the strain under ideal conditions, utilizing recommended medium, temperature and light.  Prepare a concentrated cell suspension, after good growth is achieved.  If grown in broth, pellet the cells by centrifugation.  Decant the supernatant and resuspend the pellet in fresh #616 broth using 1/10 or less of the original volume.  For slant cultures, wash cells off the agar surface with a minimal amount of #616 broth so that a concentrated cell suspension is attained.  Add 50% DMSO solution to the concentrated cell suspension so that the final concentration of DMSO in the suspension is 5%.  Dispense small aliquots (0.5 to 1 mL) of the suspension into small sterile vials.  Store the vials at -50°C or below.

When needed, remove vials from storage, thaw contents in a 37°C water bath and inoculate into recommended medium.  A minimum of 0.2 mL of the thawed stock should be used to inoculate 5 mL of broth or 1 agar slant.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

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