平台编号：Bio-134775

 Human rhinovirus 37 拉丁名

VR-1607 编号

Product category Viruses

Product type Animal virus

Classification Picornaviridae, Rhinovirus, Human rhinovirus B

Strain designation 151-1

Genome sequenced strain Yes

Isolation source Presumed from human throat washings; this preparation has been derived from VR-1147 following treatment to remove mycoplasma.

Applications Infectious disease research

Respiratory disease research

Product format Frozen

Storage conditions -70°C or colder 

Specific applicationsRespiratory research

CommentsThis product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-169-001-021 (ATCC VR-1147). It replaces VR-1147 and VR-510, which are no longer available. Strain 151-1 is the prototype strain of Human rhinovirus 37 and it has been fully sequenced (GenBank: EF173423.1).

Mycoplasma contaminationNot detectedHostH1HeLa (ATCC CRL-1958)

Alternate Hosts: WI-38 (ATCC CCL-75 ), HeLa (ATCC CCL-2 ), KB (ATCC CCL-17 ) and other human cells

Effectscell rounding; cell sloughing; CPE

Complete mediumEMEM (ATCC® 30-2003™) + 2% FBS (ATCC® 30-2020™)

Temperature33°C

Atmosphere95% Air, 5% CO2

IncubationIncubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired. Freeze preparation as rapidRecommendation for infectionFor best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture.  Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired.  Freeze preparation as rapidly as possible.Handling notes This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-169-001-021 (ATCC® VR-1147™). It replaces VR-1147™ and VR-510™, which are no longer available. Strain 151-1 is the prototype strain of Human rhinovirus 37 and this ATCC product has been fully sequenced. GenBank: EF173423.1

Key abbreviations°C, Degrees Celsius

CO2, Carbon dioxide

CPE, Cytopathic effect

EMEM, Eagle's Minimum Essential Medium

FBS, Fetal bovine serum

DepositorsNIAID, ATCCType of isolateHuman