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Lactobacillus coryniformis subsp. torquens Abo-Elnaga and Kandler

25600™

Bacterial type strain isolated from air of dairy barn

Product category

Bacteria

Strain designation

[30]

Type strain

Yes

Isolation source

Air of dairy barn

Product format

Freeze-dried

Storage conditions

2°C to 8°C

General

Preceptrol

No

Handling information

Medium

ATCC Medium 416: Lactobacilli MRS Agar/Broth

Temperature

37°C

Atmosphere

95% Air, 5% CO2

Handling procedure

Open vial according to enclosed instructions.

From a single tube of #416 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.

Aseptically transfer the rehydrated pellet back into the broth tube.  Mix well

Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.

Incubate tubes and plate at 37°C in an atmosphere of 5% CO2 for 24 to 48 hours.  Loosen screw caps of all test tubes during the incubation period.

Handling notes

After 24 to 48 hours, growth is evident by turbidity in the broth and the formation of small colonies on the slant and/or plate.  Colonies are entire, glistening, and smooth.  The CO2 atmosphere is not necessary for growth but may enhance it.

Additional information on this culture is available on the ATCC web site at www.atcc.org.

History

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