平台编号：Bio-134600

 Malassezia slooffiae J. Guillot, Midgley et E. Guého   拉丁名

38593™  统一编号

An ampoule containing viable cells suspended in cryoprotectant.

Product category  产品类别  Fungi

Product type  产品类型  Yeast

Strain designation  别名  683

Type strain 模式菌种  No

Isolation source  隔离源  Scalp

Product format  规格  Freeze-dried

Storage conditions  保存条件  2°C to 8°C

Specific applications：Biomedical Research and Development Material

Preceptrol：No

Comments：Occurrence in children

Medium 培养基

ATCC Medium 1072: Pityrosporum medium

ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth

ATCC Medium 2737: Modified Leeming & Notman Agar (MLNA)

Temperature  培养温度  37°C

Atmosphere 需氧条件 Aerobic

Handling procedure

For freeze-dry (lyophilized) ampoules:

1 Open an ampoule according to enclosed instructions.

2 From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.

3 Aseptically transfer the suspension back into the test tube of sterile distilled water.

4 Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.

5 Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.

6 Incubate the inoculum at the propagation conditions recommended.

7 Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.