Botrytis cinerea Persoon

46522™

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.

Product category

Fungi

Strain designation

LB 1

Type strain

No

Isolation source

strawberry

Applications

Agricultural research

Product format

Freeze-dried

Storage conditions

2°C to 8°C

General

Preceptrol

No

Handling information

Medium

ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth

ATCC Medium 325: Malt extract agar (Blakeslee's formula)

ATCC Medium 336: Potato dextrose agar (PDA)

Temperature

24-26°C

Atmosphere

Aerobic

Handling procedure

For freeze-dry (lyophilized) ampoules:

Open an ampoule according to enclosed instructions.

From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.

Aseptically transfer the suspension back into the test tube of sterile distilled water.

Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.

Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.

Incubate the inoculum at the propagation conditions recommended.

Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.