平台编号：Bio-134303

Aspergillus niger van Tieghem

13497™

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.

Product category

Fungi

Strain designation

Armbruster C-14

Type strain

No

Genome sequenced strain

Yes

Isolation source

UV mutant derived from M 370, ATCC 13496

Applications

Antibiotic production

Product format

Freeze-dried

Storage conditions

2°C to 8°C

General

Specific applications

produces glucoamylase

Preceptrol

No

Characteristics

Comments

Genome sequencing strain (Joint Genome Institute, Department of Energy, USA)

Technical information

ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Medium

ATCC Medium 200: YM agar or YM broth

ATCC Medium 325: Malt extract agar (Blakeslee's formula)

ATCC Medium 336: Potato dextrose agar (PDA)

Temperature

24-26°C

Atmosphere

Aerobic

Handling procedure

For freeze-dry (lyophilized) ampoules:

Open an ampoule according to enclosed instructions.

From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.

Aseptically transfer the suspension back into the test tube of sterile distilled water.

Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.

Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.

Incubate the inoculum at the propagation conditions recommended.

Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.