平台编号：Bio-134268

Rickettsia monacensis拉丁名   VR-1928™统一编号

Type strain. Genome sequenced by depositor.

Product category  Bacteria

Classification  Rickettsiaceae, Rickettsia

Strain designation  IrR/Munich

Type strain  Yes

Genome sequenced strain  Yes

Isolation source  Tick (Ixodes ricinus), isolated on June 12, 1998.

Geographical isolation  Germany; Munich

Applications  Infectious disease research

Vector-borne disease research

Product format   Frozen

Storage conditions  Vapor phase of liquid nitrogen

Comments

Genome sequenced by depositor

Handling information

Host

Vero (ATCC CCL-81)

Effects

CPE; cell rounding; cell sloughing; cell degeneration

Complete medium

DMEM (ATCC® 30-2002™) + 5% FBS (ATCC® 30-2020™)

Temperature

34°C

Incubation

6-11 days at 34°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer. A Giemsa stain may also be used to determine end of incubation.

Recommendation for infection

Plate cells 18-24 hours prior to infection and infect when cultures are 65-75% confluent. Remove DMSO from bacteria vial by centrifugation (180 x g for 10 minutes at 4°C). Remove supernatant and add 2 mL of fresh medium to the pellet. Pulse vortex for 1 minute. Remove medium from host cell culture and inoculate with bacteria (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1 to 1). Adsorb by centrifugation for 30 minutes at 800 x g at 22°C. Add agent growth medium following adsorption.

Key abbreviations

°C, Degrees Celsius

DMSO, Dimethyl sulfoxide

EMEM, Eagle's Minimum Essential Medium

FBS, Fetal bovine serum

MOI, Multiplicity of infection

Quality control specifications

Volume

1.0 mL

History

Deposited as

Rickettsia monacensis

Depositors

T Kurtti, University of Minnesota

Chain of custody

ATCC <-- T Kurtti

Type of isolate

Arthropod

Year of origin

1998

Cross references

GenBank NZ_LN794217.1 Rickettsia monacensis, strain IrR/Munic, complete genome.