平台编号：Bio-132689

KEL FIB

CRL-1762™

KEL FIB is a cell line exhibiting fibroblast-like morphology that was isolated from the connective tissue of a Black, 35-year-old, female patient’s skin with keloid. This cell line was deposited by M Babu, RF Diegelmann, I Cohen.

Product categoryHuman cells

OrganismHomo sapiens, human

MorphologyfibroblastTissueSkin; Connective tissueDiseaseKeloid

Applications3D cell culture

Product formatFrozenStorage conditionsVapor phase of liquid nitrogen

Growth propertiesAdherentAge35 yearsEthnicityBlackGenderFemaleGenes expressedcollagenCommentsProgeny from the current distribution stock (passage 11) have been shown to undergo at least 10 population doublings from the frozen state.

Unpacking and storage instructionsCheck all containers for leakage or breakage.

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete mediumThe base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).

Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

Transfer the vial contents to a centrifuge tube containing  9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.

Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 or a 75 cm2 culture flask.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medi