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Corynebacterium diphtheriae (Kruse) Lehmann and Neumann 

ATCC 27010™ 

Type strain Product category Bacteria 

Strain designation C7 s (-) tox- [NCTC 11397] 

Type strain Yes 

Isolation source Indicator strain lysed by Barksdale's phage strains gamma tox- and beta tox + (carried by lysogenic Corynebacterium diphtheriae ATCC 27011 and ATCC 27012). 

Product format Freeze-dried 

Storage conditions 2°C to 8°C 

Preceptrol No

Medium ATCC Medium 611: PGT medium 

Temperature 37°C 

Atmosphere Aerobic 

Handling procedure Open vial according to enclosed instructions. 

From a single tube of #611 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette and use to rehydrate the entire pellet. 

 Aseptically transfer the rehydrated pellet back into the broth tube. Mix well. 

 Use several drops of this suspension to inoculate an additional broth tube, an agar slant and/or a plate. 

Incubate the tubes and plate at 37°C for 24 to 48 hours. 

Deposited as Corynebacterium diphtheriae (Kruse) Lehmann and Neumann Depositors WL Barksdale Patent depository This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. 

This material may not have been produced or characterized by ATCC. 

As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims. 

Cross references GenBank AX109539 Sequence 272 from Patent WO0123604. 

GenBank AX109929 Sequence 662 from Patent WO0123604. 

GenBank AX111485 Sequence 2218 from Patent WO0123604.

GenBank AX111506 Sequence 2239 from Patent WO0123604. 

GenBank AX111526 Sequence 2259 from Patent WO0123604.