平台编号：Bio-132189

HuT 102

TIB-162.1™

Product categoryHuman cells

OrganismHomo sapiens, human

Cell typecutaneous T lymphocyteMorphologylymphoblastTissuePeripheral bloodDiseaseLymphoma

Applications3D cell culture

Immunology

Product formatFrozenStorage conditionsVapor phase of liquid nitrogen

Growth propertiesSuspension, multicellular aggregatesDerivationThe Hut 102 cell line was derived from the peripheral blood of a 26 year old Black male patient with mycosis fungoides.

Age26 yearsEthnicityBlackGenderMaleAntigen expressionCD4; Homo sapiens

Genes expressedhuman T cell leukemia virus (HTLV-I)Expression markersInterleukin 2 (IL-2)CommentsThis cell line has the properties of a mature human T cell with helper/inducer activity.

It has receptors for IL-2, and the growth rate is stimulated by IL-2.

This cell line is E-rosette positive (E+), and negative for Epstein-Barr virus nuclear antigen (EBNA-), surface immunoglobulin (sIg-) and terminal deoxynucleotidyl transferase (TdT-).

The cell line was originally thought to produce IL-2; however, recent studies have failed to detect IL-2 mRNA production in Hut 102 cells.

TIB-162.1 is derived from TIB-162. It has been observed that culturing TIB-162 in culture media, RPMI-1640,  containing FBS (10%) alone could not support a desired cell growth outcome. However, adding IL-2 into the recommended culture media significantly increases cell survival and proliferation. So TIB-162.1 is cultured in RPMI1640 Medium supplemented with 10% FBS, and 100 U/ml IL-2.

Unpacking and storage instructionsCheck all containers for leakage or breakage.

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete mediumThe base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001). To make the complete growth medium, add the following components to the base medium:  

Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 10% 

100 U/mL IL-2

Temperature37°C

Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).

Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

Transfer the vial contents to a 75 cm2 tissue culture flask and dilute with the recommended complete culture medium (see the specific batch information for the recommended dilution ratio).   It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.

If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension