平台编号：Bio-110018

 Paxillus involutus (Batsch : Fries) Fries拉丁名  

(ATCC® MYA-4647™) 统一编号 

Strain Designations 别名 ATCC 200175 [87.017]

Biosafety Level 生物安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 frozen

Storage Conditions 保藏条件 Frozen: -80°C or colder

Freeze-Dried: 2°C to 8°C

Live Culture: See Propagation Section

Type Strain no

Preceptrol® 模式菌株 no

Genome Sequenced Strain Yes

Comments 注释

Amino acid uptake.

Cadmium uptake.

Poisonous mushroom.

Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA).

Morphology On YM agar, after 35 days at 25°C, colonies are white to creamy, velutinous, dense, radially sulcate, and exudate is brown to dark brownin color. Clamp connections present on hyphae, spores not observed.

Medium ATCC® Medium 200: YM agar or YM broth

ATCC® Medium 28: Emmons' modification of Sabouraud's agar

ATCC® Medium 2798: MMNC Medium

Growth Conditions

Temperature: 24°C to 26°C

Atmosphere: Typical aerobic

Sequenced Data

18S ribosomal RNA gene, partial sequence; internal   transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.

CATTATCGAAAAGCAATCCGGGGGGAGGCGACCGAGCGAAGTTTGGTAGATCGTAGGGATTGTCGCTGGCCTTTGGAAACGAAGGCATGTGCACGTTCCGAGTTCTCCTTAGTCCTCCTTTGCCTTTCCTTTGGAAAACCCTTTCTTCACACCCGTGCACCCATTGTAGGTCTCCGCGAGGGGATCTATGTCTTCACATAAACACTACGTATGTCTAGGAATGTATCTAAAAGCGTCGGACGGCTTGGCTTCGTGCCCGGTCGGCGACGGTAAAGAACCATAATACAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAACGCAGCGAATTGCGATAAGTAATGTGAATTGCAGATTTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTTAATTCTCAACCATCCCTCGATTCGTTTCGAGGGTTTGGCTTGGATTTTGGGGGCTGCCGGGCGACCCTAGGGTCTTCGGCTCTCCTTGAAAGCATTAGCGATGGCGGCGCGATCTAACCCCCCC

D1D2 region of the 28S ribosomal RNA gene

CATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACTGCGAGTGAAGCGGGATGAGCTCAAATTTCGAATCTGGCGGTCTTCAGGCCGTCCGAGTTGTAATCTAGAGAAGCGTCTTCCGCGCTGGACCGTGTACAAGTCTCCTGGAAGGGAGCGTCGTAGAGGGTGAGAATCCCGTCTTTGACACGGACTGCCAGTGCTATGTGATGCGCTCTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCAAATCGGGTGGTAAACTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGAACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGCTGAAAGGGAAACGCTTGAGGTCAGTCGCGTCGGACGGGGATCAACCTTGCTTCTCGCTCGGTGTACTTCCTGCTCGACGGGTCAGCATCAGTTTCGGTCGCCGTACAAGGGTCGAGGGAATGTGGCACTCCTCGGAGTGTGTTATAGCCTTCGGTCGCATGCGGTGGTTGGGACTGAGGAACTCAGCACGACCCCTCGAGGGTTCGGGGCCACGGCCTACGTTAACGTGCTTAGGATGCTGGCATAATGGCCTTAAGCGA

Morphology On YM agar, after 35 days at 25°C, colonies are white to creamy, velutinous, dense, radially sulcate, and exudate is brown to dark brownin color. Clamp connections present on hyphae, spores not observed.

Name of Depositor T Johansson

Special Collection ATCC

Chain of Custody

ATCC <-- T Johansson

Isolation

Birch plantation, Bush Estate, Penicuik, Midlothian, Scotland.

Cross References

Nucleotide (GenBank) : HQ999961 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : JOMD00000000 Paxillus involutus ATCC 200175, whole genome shotgun sequencing project

References

Kohler A, et al. Convergent losses of decay mechanisms and rapid turnover of symbiosis genes in mycorrhizal mutualists. Nat Genet 47: 410-415, 2015. PubMed: 25706625

Blaudez D, et al. Cadmium uptake and subcellular compartmentation in the ectomycorrhizal fungus Paxillus involutus. Microbiology 146: 1109-1117, 2000. PubMed: 10832638

Chalot M, et al. Kinetics, energetics and specificity of a general amino acid transporter from the ectomycorrhizal fungus Paxillus involutus. Microbiology 142: 1749-1756, 1996.

Chalot M, et al. Factors affecting amino acid uptake by the ectomycorrhizal fungus Paxillus involutus. Mycol. Res. 99: 1131-1138, 1995.