平台编号：Bio-107015

Trichoderma atroviride Karsten sensu Bissett (ATCC® 36042™)

Deposited AsTrichoderma lignorum (Tode) Harz, anamorph

Strain Designations2440A [CBS 391.92]

Alternate StateHypocrea atroviridis Dodd et al.

Applicationproduces peptaibol antibiotics

Biosafety Level1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Formatfreeze-dried

Type Strainno

Preceptrol® no

Comments postharvest decay of citrus fruit

Morphology After 12 days on Blakeslee’s malt extract medium at 25°C, colony is low to floccose, mycelium white to yellow, conidial areas green, reverse uncolored. Chlamydospores globose, hyaline, intercalary or terminal on short branches. Conidiophores hyaline, with pyramidal branching, phialides in terminal verticils. Conidia subglobose, smooth-walled, green.MediumATCC® Medium 325: Malt extract agar (Blakeslee's formula)

ATCC® Medium 336: Potato dextrose agar (PDA)

Growth ConditionsTemperature: 24°C

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence.GGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACCATACCAAACTGTTGCCTCGGCGGGGTCACGCCCCGGGTGCGTCGCAGCCCCGGAACCAGGCGCCCGCCGGAGGGACCAACCAAACTCTTTTCTGTAGTCCCCTCGCGGACGTTATTTCTTACAGCTCTGAGCAAAAATTCAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGACCTCGGGAGCCCCTAAGACGGGATCCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCGCGTCCACGTCCGTAAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAAC

Morphology After 12 days on Blakeslee’s malt extract medium at 25°C, colony is low to floccose, mycelium white to yellow, conidial areas green, reverse uncolored. Chlamydospores globose, hyaline, intercalary or terminal on short branches. Conidiophores hyaline, with pyramidal branching, phialides in terminal verticils. Conidia subglobose, smooth-walled, green.

Name of Depositor D Knosel

Isolation 

lemon

Cross References 

Nucleotide (GenBank) : AF049075 Trichoderma harzianum internal transcribed spacer 1, partial

Nucleotide (GenBank) : U05192 Trichoderma harzianum orotidine-5'-phosphate decarboxylase (pyr4)

Nucleotide (GenBank) : AF276093 Trichoderma atroviride strain ATCC 36042 small subunit ribosomal

Nucleotide (GenBank) : AF278796 Trichoderma atroviride strain ATCC 36042 18S ribosomal RNA gene,

Nucleotide (GenBank) : AF276651 Trichoderma atroviride strain ATCC 36042 endochitinase 42 (ech42)

Nucleotide (GenBank) : Z48812 internal transcribed spacers, ITS-1 and ITS-2, and the 5.8S ribosomal DNA, sequences

References 

. . Phytopathol. Z. 85: 217-226, 1976.

Schirmbock M, et al. Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi. Appl. Environ. Microbiol. 60: 4364-4370, 1994. PubMed: 7811076

Ospina-Giraldo MD, et al. Phylogenetic relationships of Trichoderma harzianum causing mushroom green mold in Europe and North America to other species of Trichoderma from world-wide sources. Mycologia 90: 76-81, 1998.