平台编号：Bio-105113

Candida broadrunensis Suh et Zhou 拉丁名

ATCC MYA-4650 编号

Strain Designations 菌种别名 EH019 [CBS 11838]

Application Ferments cellobiose

Biosafety Level 安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 frozen

Storage Conditions 培养条件

Frozen 冷冻物 : -80°C or colder

Freeze-Dried 冻干物 : 2°C to 8°C

Live Culture: See Propagation Section

Type Strain 模式菌株 yes

Preceptrol no

Comments 注释 Insect associated yeast

Morphology After 10 days in YM broth at 25°C, cells are subglobose to ellipsoidal (2-4 x 2-4 ?m) and occur singly, in pairs or in small clusters. Pseudohyphae and true hyphae are not present. After 10 days on YM agar at 25°C, colonies are white to cream-colored, butyrous and smooth with entire margins.

Medium 培养基 ATCC Medium 28: Emmons' modification of Sabouraud's agar

ATCC Medium 200: YM agar or YM broth

ATCC Medium 1245: YEPD

Growth Conditions 生长条件

Temperature 培养温度 : 20°C to 25°C

Atmosphere 需氧情况 : Typical aerobic

Sequenced Data 

ITS DNA sequence of MYA-4650:

TGATTTGAGGTCAAACTTGTTTGTTGTTTTGTAAGGCAAAGCCAAATATACCAAAAATTCACTAACTGGAATTCTCAACGAGTTGGATAAACCTAATACATTGAAAATGTTCCTGAAAGCACTATTTAGTACGACTCGTGCCAATACTTTTCAAGCAAACGCCTAGTTCGACTAAGAGTATCACTCAATACCAAACCCGAAGGTTTGAGAGAGAAATGACGCTCAAACAGGCATGCCCTTTGGAATACCAAAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGAAAATCTGCAATTCATATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTTTGAAGATTTTTGAATTTAATCAACAAATTGACAAATAATAAATAAACAATTCAATTAAAAATTGAAGTTTTAAACCTCTGGCCCAACTTATTTCTAAGCCAGACCAAAGCAAAAGTTTCTAATAAAGAAAAACATTGTGTGTAAGTTTGTTTACCGCGCAATTAAGCGCAGGT

D1D2 region of the 26S ribosomal RNA gene:

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGTCTTAGTAACGGCGAGTGAAGCGACAAAAGCTCAAATTTGAAATCTGGCGCCTTCGGTGTCCGAGTTGTAATTTGAAGAAGGTAACTTTGGTTTTGGCTCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGCCCAATTCCATGTAAAGTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCTTGAGATCAGACTTGGTATTTTGTATGTCTTACTTTCGGGTGGGGCCTCTGCAGTTTACCGGGCCAGCATCAGTTTGGATGGTAGGATAATGACATTGGAATGTGGCTCTGCTTCGGTAGAGTGTTATAGCCCTTGTTGATACTGCCTGTCTAGACTGAGGACTGCGTCTTTGACTAGGATGCTGGCATAATGATCCTAAGCCAC

Morphology After 10 days in YM broth at 25°C, cells are subglobose to ellipsoidal (2-4 x 2-4 ?m) and occur singly, in pairs or in small clusters. Pseudohyphae and true hyphae are not present. After 10 days on YM agar at 25°C, colonies are white to cream-colored, butyrous and smooth with entire margins.

Name of Depositor 寄存者 SO Suh

Special Collection ATCC

Chain of Custody 来源国家 ATCC <-- SO Suh

Isolation 分离基物

Gut of unidentified tenebrionid beetle from rotten log, Bull Run Mt., Broad Run, Virginia, USA.

Cross References 

Nucleotide (GenBank) : HQ651969 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : HQ652048 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : HQ651934 18S rRNA gene

Nucleotide (GenBank) : KC507462 EF-1 alpha gene

Nucleotide (GenBank) : KC507422 RPB1 gene

Nucleotide (GenBank) : KC512847 RPB2 gene

Nucleotide (GenBank) : KC479715 XYL1 gene

References 参考文献

Suh SO, et al. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov., and Candida manassasensis sp. nov., four novel yeasts associated with wood- ingesting insects and their ecological and biofuel implications. Int. J. Syst. Evol. Microbiol. 63: 4330-4339, 2013. PubMed: 24014624