平台编号：Bio-104471

Gibberella fujikuroi (Sawada) Wollenweber 拉丁名

(ATCC? 12616?) 统一编号

Strain Designations 别名 IMI 58290 [917, CBS 183.29, DSM 893]

Alternate State Fusarium moniliforme Sheldon, anamorph

Application 用途 Produces gibberellic acid GA3, gibberellin A3

Produces gibberellins

Transformation of ent-15beta-hydroxy-kaur-16-ene derivatives

Transformation of ent-15beta,16beta-epoxy-kaurane derivatives

Biosafety Level 生物安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 frozen

Storage Conditions 保藏条件Frozen: -80°C or colder

Freeze-Dried: 2°C to 8°C

Live Culture: See Propagation Section

Type Strain 模式菌株 no

Preceptrol? no

Comments 注释 Nonsporulating

Regulation of secondary biosynthesis

Medium 培养基 ATCC? Medium 28: Emmons' modification of Sabouraud's agar

ATCC? Medium 307: Cornmeal agar

ATCC? Medium 336: Potato dextrose agar (PDA)

Growth Conditions 生长条件

Temperature 培养温度: 24°C to 26°C

Atmosphere 需氧情况: Typical aerobic

Sequenced Data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

AGGGATCATTACCGAGTTTACAACTCCCAAACCCCTGTGAACATACCAATTGTTGCCTCGGCGGATCAGCCCGCTCCCGGTAAAACGGGACGGCCCGC

CAGAGGACCCCTAAACTCTGTTTCTATATGTAACTTCTGAGTAAAACCATAAATAAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGAT

GAAGAACGCAGCAAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGG

CATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCCCCGGGTTTGGTGTTGGGGATCGGCGAGCCCTTGCGGCAAGCCGGCCCCGAAATCTAGTGGCG

GTCTCGCTGCAGCTTCCATTGCGTAGTAGTAAAACCCTCGCAACTGGTACGCGGCGCGGCCAAGCCGTTAAACCCCCAACTTCTGAATGTTGACCTCG

GATCAGGTAGGAATACCCGCTGAACTTAAG

D1D2 region of the 28S ribosomal RNA gene

CATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTCTCGGGC

CCGAGTTGTAATTTGTAGAGGATACTTTTGATGCGGTGCCTTCCGAGTTCCCTGGAACGGGACGCCATAGAGGGTGAGAGCCCCGTCTGGTTGGATG

CCAAATCTCTGTAAAGTTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATGTCTTCTAAAGCTAAATACCGGCCAGAG

ACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGTACGTGAAATTGTTGAAAGGGAAGCGTTTATGACC

AGACTTGGGCTTGGTTAATCATCTGGGGTTCTCCCCAGTGCACTTTTCCAGTCCAGGCCAGCATCAGTTTTCCCCGGGGGATAAAGACTTCGGGAATG

TGGCTCTCTTCGGGGAGTGTTATAGCCCGTTGTGTAATACCCTGGGGGGGACTGAGGTTCGCGCATCTGCAAGGATGCTGGCGTAATGGTCATC

AACGA

Name of Depositor IMI

Chain of Custody ATCC <-- IMI <-- P.W. Brian 917

Isolation 分离基物 Rice culm, Oryza sativa, Japan

References 参考文献 Munoz GA, Agosin E. Glutamine involvement in nitrogen control of gibberellic acid production in Gibberella fujikuroi. Appl. Environ. Microbiol. 59: 4317-4322, 1993.

Fraga BM, et al. Biotransformation of two ent-15?-hydroxy-kaur-16-ene derivatives by Gibberella fujijuroi. Phytochemistry 31: 3845-3849, 1992.

Saucedo JE, et al. Continuous production of gibberellic acid in a fixed-bed reactor by immobilized mycelia of Gibberella fujikuroi in calcium alginate beads. Appl. Microbiol. Biotechnol. 30: 226-233, 1989.

Harris GM. Protoplasts from Gibberella fujikuroi. Phytopathology 72: 1403-1407, 1982.

Bu'lock JD, et al. Regulation of secondary biosynthesis i Gibberella fujikuroi. Trans. Br. Mycol. Soc. 62: 377-389, 1974.

Darken MA, et al. Production of gibberellic acid by fermentation. Appl. Microbiol. 7: 301-303, 1959.