平台编号：Bio-104470

Cryptococcus gattii (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout 拉丁名

(ATCC? MYA-4877?) 统一编号

Strain Designations 别名 A6MR38 [CBS 11545]

Alternate State Filobasidiella bacillispora Kwon-Chung, teleomorph

Biosafety Level 生物安全等级 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 frozen

Type Strain no

Genotype VGIIc; AFLP6C

Preceptrol? no

Mating Type MATalpha

Comments 注释 highly virulent novel genotype Ref

Morphology On YM medium at 25°C after 6 days, colonies creamy white to pale yellow, mucoid. Cells globose to subglobose, smooth, contain oil droplets, 3-5.25 ?m X 3-5.25 ?m. Pseudohyphae not observed.

Medium 培养基 ATCC? Medium 200: YM agar or YM broth

ATCC? Medium 1245: YEPD

Growth Conditions 生长条件

Temperature 培养温度: 20°C to 25°C

Atmosphere 需氧情况: Typical aerobic

Sequenced Data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.

GTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATACTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATG

TGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACAT

CGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAA

GGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCAGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTC

GGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACA

ACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAA

D1D2 region of the 28S ribosomal RNA gene:

ACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGA

AACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGA

TACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAAC

AAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTA

TTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGG

GGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTG

ACAAAATGGCTTTAAACGACCCGTCTT

Morphology On YM medium at 25°C after 6 days, colonies creamy white to pale yellow, mucoid. Cells globose to subglobose, smooth, contain oil droplets, 3-5.25 ?m X 3-5.25 ?m. Pseudohyphae not observed.

Name of Depositor CBS

Special Collection ATCC

Chain of Custody ATCC <-- CBS <-- K Bartlett, E Burne, F Hagen

Isolation

CSF of immunicompetent human, Oregon, USA

Cross References

Nucleotide (GenBank) : JQ812708 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : JQ812702 D1/D2 region of LSU rRNA gene

References

MacDougall, L., et al. Spread of Cryptoccus gattii in British Columbia, Canada, and detection in the Pacific Northwest, USA. Emerg. Infect. Dis. 13(1): 42-50, 2007. PubMed: 17370514

Byrnes EJ III, et al. Emergence and pathogenicity of highly virulent Cryptococcus gattii genotypes in the Northwest United States. PLoS Pathog. 6: e1000850, 2010. PubMed: 20421942

Byrnes EJ III, et al. Molecular evidence that the Vancouver Island Cryptococcus gattii outbreak has expanded into the United States Pacific Northwest. J. Infect. Dis. 199: 1081-1086, 2009. PubMed: 19220140

Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334