ProLink Express- Fully Automated Proteomics Workcell
The ProLink
Express represents a breakthrough in integrating laboratory instruments
that has changed the way researchers use automation. It is the first
fully automated proteomics workcell. that integrates all plate delivery
and transfer operations with complete data tracking. It automates the
entire protein expression process, from initial bacterial colony picking
through final protein expression and measurement. The ProLink Express
system is capable of delivering a plate to any of its instruments from
any other instrument, in any order and simultaneously with other
processes.
The ProLink Express is designed so that colony picking,
growth, DNA prep and expression can be run individually or sequentially.
All instrumentation faces outward so any component can be used
manually. It is powered by our opportunistic SoftLinx scheduling
software, which finds the most efficient ways to perform processes
simultaneously. A user can run multiple batches in different stages of
the process at the same time, or run any instrument individually while
operating another, fully automated run.
The ProLink Express
integrates an automated colony picker with an incubator and a plasmid
DNA prep system. A researcher can start with bacterial colony plates,
isolate individual colonies into culture plate wells, grow the
individual colonies and then extract the DNA plasmids in one seamless
automated process without any human intervention. The system also
provides the capability to re-attach DNA inserts onto expression vectors
and perform in vitro or in vivo protein expression and detection.
The
features and benefits of the ProLink Express make it simply remarkable –
and offer multiple application opportunities within molecular biology.
The ProLink Express gives the researcher an unprecedented level of
flexibility and modularity not available from any other integrated
laboratory system on the market today.
Protein Expression: The
ProLink Express is the world’s first FULLY automated proteomics
workcell. This unique system is the first robot system to integrate all
plate-delivery and transfer operations, together with fully-automated
data tracking, from initial bacterial colony picking all the way through
final protein expression and measurement. ProLink Express links
together all the components needed for DNA expression of gene libraries.
It will enable automated gene expression for functional genomics, gene
improvement or any other type of genomic research involving bacterial
cultures, DNA prep and protein expression.
Gene Assembly: ProLink
Express’ inherent plate delivery, liquid handling, thermocycler and
absorbance reader instruments provide PCR gene-assembly capacity with a
throughput greater than that of any other system today, thanks to
SoftLinx software’s ability to process multiple plates through different
stages of the process simultaneously.
Monoclonal Antibody
Development and Production: The ProLink Express is ideal for monoclonal
antibody development. ProLink ‘s remarkable capacity to perform multiple
processes simultaneously will rapidly, and accurately, identify and
isolate hybridomas producing desired monoclonal antibodies. The ProLink
Express will process these cells through the elaborate series of
repetitive steps required to statistically isolate the desired
hybridomas automatically, keeping track in its database of every sample
in every well throughout the process.
The ProLink Express relieves
technicians of the burden of what is usually a mind-numbing, error-prone
process of repeated dilutions and analyses of the multiplexed hybridoma
population to eventually get them down to a single type in a microplate
well through dilution cloning.
ELISAs: With its in-line
room-temperature and controlled-temperature incubators, reagent
dispensers, pipettors, plate-handlers and plate readers (including
seal-piercing and/or lid-handling capability), the ProLink Express is
ready-made to perform ELISAs of nearly any description or complexity.
Multiple incubation steps and complicated reagent and conjugate
requirements are no problem for the multi-tasking flexibility or the
ProLink Express.
Protein Expression & Purification
Hudson
offers a wide range of instrumentation to support the entire range of
protocols commonly encountered when preparing peptides and proteins.
From liquid handlers to support PCR through vast, multi-component
systems to support an entire research effort.
The ProLink Express
links all the components needed for DNA expression of gene libraries. It
enables automated gene expression for functional genomics, gene
improvements and any other type of genomic research involving bacterial
cultures, DNA prep and protein expression. The ProLink Express has many
valuable features not found in other systems:
Optimizes laboratory processes and equipment usage
Designed to fit into available lab space
Allows independent as well as manual access to and operation of all major system instruments
Capable
of delivering a plate to any of its instruments from any other
instrument, in any order and simultaneously with other processes
Includes
Hudson Robotics’s SoftLinx software, which provides an easy-to-use
interface to coordinate the ProLink Express? operation with other
automated instruments; the software will enable the user to take full
advantage of this system feature, which is unique in the lab automation
industry.
Hudson’s ProLink Express is a modular, flexible automation
system that permits its users to include or bypass any instrument or
instrument group within any method the user may wish to run. Taking
advantage of SoftLinx’ extensive library of third-party instrument
drivers, virtually any robot-compatible lab instrument on the market can
become part of a ProLink system. ProLink is fully scalable, so that
users can start with small workcells then expand them to include more
instruments in a growing automation system, yet without sacrificing the
usefulness and simplicity of each original smaller workcell.
Typical functional subsystems include:
Colony-picking, sealing and cell outgrowth
Cell pelletting, media aspiration and re-suspension
DNA prep, purification and yield measurement
PCR reaction preparation and running
Transformations/transfections and cell recovery
In vivo/in vitro protein expression
Protein purification and measurement
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