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GATTA-荧光小球 / 超高分辨率STED显微镜测试工具
SMALL, BRIGHT AND CUTTING-EDGE. EXPERIENCE THE HIGHEST BRIGHTNESS DENSITY IN THE WORLD.
Fluorescent beads are important microscopy tools which can be used for calibration of microscopes, particle tracking or quantitative determination of point spread functions especially in STED microscopy. Particularly for the last point a small structure size keeping a high brightness is an immense advantage. Products from the GATTA-Bead series fulfill this requirement perfectly with a diameter of only 23nm. Additionally, they show a clearly improved homogeneity and flexibility compared to other beads. We offer GATTA-Beads in the colors red (ATTO 647N), orange (ATTO 594), green (ATTO 542) and blue (Oregon Green 488). Moreover, we offer alternative dyes which are indicated on the table below
应用范围:
· 系统设置校准
· 2D STED测量
· 3D STED PSF 反卷积
· 跟踪及漂移校准
优势:
· 大小: 直径 = 23 nm
· 亮度: 高亮，高密度
· 高度均一: 超级小的尺寸及强度分布
· 灵活性: 颜色修改或者增加化学成分
GATTA-荧光小球染料特性
Bead R | Bead O | Bead G | Bead B | |
颜色 | 红色 | 桔色 | 绿色 | 蓝色 |
可用染料 | ATTO647N | ATTO 594 | ATTO 542 | Oregon Green 488 |
可替代染料 | Abberior STAR 635P | - | Cy3 or Alexa Fluor 555 | Alexa Fluor 488 or ATTO 490LS |
推荐激光源 | 630-650 nm | 565-610 nm | 515-540 nm | 480-505 nm |
尺寸 | 23 nm | 23 nm | 23 nm | 23 nm |
表面密度 | ≈ 1/μm2 | ≈ 1/μm2 | ≈ 1/μm2 | ≈ 1/μm2 |
FLUOROPHORE PROPERTIES荧光属性
荧光染料 | 颜色定义 | 激发光波长 / nm | 发射光波长 / nm |
ATTO 647N | R | 647 | 664 |
Abberior Star 635P | R | 635 | 651 |
ATTO 594 | O | 594 | 626 |
ATTO 542 | G | 542 | 562 |
Cy3 | G | 554 | 568 |
Alexa Fluor 555 | G | 555 | 580 |
Oregon Green 488 | B | 488 | 526 |
Alexa Fluor 488 | B | 488 | 525 |
ATTO 490LS | B | 490 | 658 |
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