Azure Cielo实时荧光定量PCR仪

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Azure Biosystems

型号

Cielo 6/3

产地

美洲美国

应用领域

暂无

Azure中国

营业执照已认证

白金会员
第3年
一般经销商

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邮件通知

灵活兼容

流程智能

可靠稳定

产品特点

邮件通知

临近下班刚开始一轮实验，且急需数据怎么办？Cielo的自动邮件通知功能帮你搞定。

通过Wi-Fi连接，Cielo Manager软件在运行结束后邮件通知，同时将数据直接发送至您邮箱。

快速设置，确认布局

每个孔均可按样本类型、生物重复和技术重复进行颜色编码。孔类型及标准曲线也直观可见。

查看单独扩增轨迹

可按指定靶标或样本，查看其单独的扩增轨迹。近红外激光器具有信号高、背景低的优势。我们高性能的多路NIR激光器和滤波器提供强大的激发能量，最大程度提高发射强度，实现最大灵敏度

5-point, 10-fold dilution series of a six-target qPCR assay. Targets: RRP36 (Efficiency = 96.8%, R2 = 0.99), ACTB (Efficiency = 97.1%, R2 = 0.99), SDHA (Efficiency = 98.0%, R2 = 0.99), GAPDH (Efficiency = 100.8%, R2 = 0.99), TBP (Efficiency = 95.5%, R2 = 0.99), and B2M (Efficiency = 97.5%, R2 = 0.99).

7-point, 10-fold dilution series in duplicate amplifying a 150 bp amplicon (Efficiency = 94.3%, R2 = 0.99), with two unknowns, each with four replicates.

样本2D热图

让您轻松查看样本，快速了解数据。

无需ROX光学校正

基于高效的全孔扫描技术，Cielo每孔扫描多个数据点，无需染料校正（如ROX）。

使用人基因组DNA模板、人GAPDH引物及无ROX SYBR Green qPCR Mix生成扩增曲线。

Amplification curves for 96 replicates of the same 150 bp amplicon (Mean Cq = 19.5, Cq Standard deviation = 0.03).

全孔扫描技术，每孔约扫描100,000个数据点

不同于竞品每孔仅扫描1个像素点，Cielo每孔约扫描100,000个像素点，从而实现每个qPCR孔荧光强度的准确、可重复和灵敏呈现。

为重现性而生

尖端工程设计，确保数据质量。珀尔帖热模块，打造卓越的热循环；无与伦比的光学设计，保障每次运行准确且可重复。

无与伦比的一致性：线性图上显示了96孔重复的扩增曲线。

(A) and a logarithmic plot (Figure B). In each well, 105 copies of GAPDH template were amplified in the presence of GAPDH primers and BioRad™ Sso Advanced SYBR Green Mix®. Average Cq = 19.1, Coefficient of variance (Cv)= 0.002.

8-point, 10-fold standard curves for six targets. Targets: RRP36 (Efficiency = 103.6%, R2 = 0.96), ACTB (Efficiency = 100.5%, R2 = 0.99), RNAse P (Efficiency = 97.0%, R2 = 0.99), GAPDH (Efficiency = 100.2%, R2 = 1.00), TBP (Efficiency = 96.8%, R2 = 0.99), and EF1a (Efficiency = 97.2%, R2 = 0.99).

6靶标多重检测

轻松使用多达6个荧光通道，染料选择灵活，应用广泛。无需Rox，可用作探针。

型号比较

Azure Cielo 6

SAMPLE CAPACITY (WELLS)

COMPATIBLE PLATES/TUBES

96-WELL PLATES & TUBES AND 8

OR 12-WELL STRIPS & SINGLE TUBES

REACTION VOLUME

1-150 uL (10-65 uL recommended)

DYNAMIC RANGE

10 LOGS

EXCITATION SOURCE

LED, EXCITATION RANGE: 450–730NM

DETECTION CHANNELS

MULTIPLEXING

UP TO 6 TARGETS

REACTION TEMPERATURE

4°C to 99.9°C

MAX. BLOCK RAMP RATE

6°C/sec

AVG. SAMPLE RAMP RATE

4°C/SEC

TEMPERATURE UNIFORMITY

±0.2°C

TEMPERATURE ACCURACY

±0.1°C

CUSTOM DYE/CHEMISTRY

✓

ONBOARD MEMORY

32GB EQUIVALENT TO 20,000 EXPERIMENTS

CONNECTIVITY

USB, WI-FI, ETHERNET

FOOTPRINT (W/D/H)

12″ X 20″ X 17″ (30.5 X 50.8 X 43.2 CM)

Azure Cielo 3

SAMPLE CAPACITY (WELLS)

COMPATIBLE PLATES/TUBES

96-WELL PLATES & TUBES AND 8

OR 12-WELL STRIPS & SINGLE TUBES

REACTION VOLUME

1-150 uL (10-65 uL recommended)

DYNAMIC RANGE

10 LOGS

EXCITATION SOURCE

LED, EXCITATION RANGE: 450–580NM AND 610–700NM

DETECTION CHANNELS

MULTIPLEXING

UP TO 3 TARGETS

REACTION TEMPERATURE

4°C to 99.9°C

MAX. BLOCK RAMP RATE

6°C/sec

AVG. SAMPLE RAMP RATE

4°C/SEC

TEMPERATURE UNIFORMITY

±0.2°C

TEMPERATURE ACCURACY

±0.1°C

CUSTOM DYE/CHEMISTRY

✓

ONBOARD MEMORY

32GB EQUIVALENT TO 20,000 EXPERIMENTS

CONNECTIVITY

USB, WI-FI, ETHERNET

FOOTPRINT (W/D/H)

12″ X 20″ X 17″ (30.5 X 50.8 X 43.2 CM)

型号比较

Azure Cielo 6

CHANNEL 1

EX: 475 ± 14

EM: 524 ± 12

DYES: SYBR™ Green, EvaGreen™, FAM™

CHANNEL 2

EX: 527 ± 10

EM: 565 ± 12

DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560

CHANNEL 3

EX: 572 ± 7.5

EM: 623 ± 12

DYES: TAMRA™, Cy3

CHANNEL 4

EX: 537 ± 13

EM: 583 ± 11

DYES: ROX™, TEX®615, CAL Fluor® Red 610

CHANNEL 5

EX: 623 ± 12

EM: 676 ± 18.5

DYES: CY®5, Quasar®670, Liz®, Mustang Purple®

CHANNEL 6

EX: 655 ± 7.5

EM: 711 ± 12.5

DYES: CY® 5.5, Quasar 705

Azure Cielo 3

CHANNEL 1

EX: 475 ± 14

EM: 524 ± 12

DYES: SYBR™ Green, EvaGreen™, FAM™

CHANNEL 2

EX: 527 ± 10

EM: 565 ± 12

DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560

CHANNEL 3

NOT AVAILABLE

CHANNEL 4

NOT AVAILABLE

CHANNEL 5

EX: 623 ± 12

EM: 676 ± 18.5

DYES: CY®5, Quasar®670, Liz®, Mustang Purple®

CHANNEL 6

NOT AVAILABLE

售后服务

保修期： 1年

是否可延长保修期：是

现场技术咨询：有

免费培训：不限次数

免费仪器保养：一年一次

保内维修承诺：保修期内免费更换配件

报修承诺： 24小时内响应

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