方案摘要
方案下载应用领域 | 医疗/卫生 |
检测样本 | 全血/血清/血浆 |
检测项目 | |
参考标准 | SMART Digest IA USERS MANUAL: https://tools.thermofisher.com/content/sfs/manuals/Man-21549-SMART-Digest-IA-User-Man21549-EN.pdf |
• The rapid processing of antibodies using the SMART Digest IA kits allows development of faster methods compared to conventional protein enrichment and digestion by simplifying sample preparation and enabling a reproducible, sensitive, and fully integrated LC/MS/MS workflow. • This application shows a rapid, simple, and sensitive method for the quantification of human IgG in mouse plasma using the SMART Digest IA kits with a total time of 3–4 hours that compares to a process that can take up to 24 hours. • The method described provides a significant simplification for the affinity proteomics process within a single well providing increased sensitivity and reduced sample preparation time. • This Application Note provides a general approach for quantifying low abundance mAbs with reproducible results across a broad dynamic range for all three types of SMART Digest (IA) kits (SMART digest IA Streptavidin, Protein A, and Protein G).
To demonstrate the rapid quantitation of a low-level human IgG in animal
plasma by LC/MS/MS using the Thermo Scientific™ SMART Digest™
Immunoaffinity (IA) kits (including magnetic and non-magnetic versions of the
Streptavidin kit, Protein A kit, and Protein G kit), which combine the IA capture
and digestion process into a single well. The assay is required to be both
selective and accurate.
• The rapid processing of antibodies using the SMART
Digest IA kits allows development of faster methods
compared to conventional protein enrichment and
digestion by simplifying sample preparation and
enabling a reproducible, sensitive, and fully integrated
LC/MS/MS workflow.
• This application shows a rapid, simple, and sensitive
method for the quantification of human IgG in mouse
plasma using the SMART Digest IA kits with a total time
of 3–4 hours that compares to a process that can take
up to 24 hours.
• The method described provides a significant
simplification for the affinity proteomics process within
a single well providing increased sensitivity and
reduced sample preparation time.
• This Application Note provides a general approach for
quantifying low abundance mAbs with reproducible
results across a broad dynamic range for all three types
of SMART Digest (IA) kits (SMART digest IA
Streptavidin, Protein A, and Protein G).
高压离子色谱Inuvion快速测定自来水中的常见阴离子
高压离子色谱Inuvion快速测定生活饮用水中的亚氯酸 盐、溴酸盐、氯酸盐、二氯乙酸和三氯乙酸
Thermo Fisher Scientific iCAP TQe ICP-MS/MS测定土壤和沉积物多种元素
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