方案摘要
方案下载| 应用领域 | 医疗/卫生 |
| 检测样本 | 全血/血清/血浆 |
| 检测项目 | |
| 参考标准 | Greaves, R.F.; Jevalikar, G.; Hewitt, J.K.; Zacharin, M.R. A guide to understanding the steroid pathway: new insights and diagnostic implications. |
A robust and rapid LC-MS method for the determination of steroids in plasma samples using an affordable HRMS instrument has been presented and is suitable for routine research analyses. Results acquired using this method demonstrate that the Q Exactive Focus MS exhibits accuracy, precision, and sensitivity that is comparable to triple quadrupole MS instruments. Various steroid isomers can be detected in full scan but also in MS/MS acquisition showing that there is a need for an efficient chromatography. In addition, with sensitive and selective full scan data, the Q Exactive Focus HRMS can be used for more in-depth investigations when requested by researchers or biochemists.
To develop a robust liquid chromatography–high-resolution mass
spectrometry (LC-HRMS) analysis for the routine quantification of steroids in
plasma that fulfills clinical research demand for sensitivity and selectivity.
Steroid hormones are endogenous metabolites that
are synthesized by various enzymes from cholesterol.
There are tens of these hormones with different effects
even at very low concentrations (nM–pM levels).1,2
Their determination can be relevant for clinical research.
Steroid determinations by immunoassays are affected by
interferences and are not fully appropriate for children
and women.
The steroid backbone of cholesterol does not contain
amino groups; therefore, various ionization sources have
been employed. However, recent routine LC-MS research
analyses of steroids (underivatized) use a 2.1 mm ID
(inner diameter) analytical column coupled with
electrospray ionization (ESI) and triple quadruople MS.
Indeed, LC-MS research methods almost exclusively use
triple-quadrupole mass spectrometry. However, many
articles have recently shown that LC-MS analysis using
high resolution (HR) technology can perform robust,
quantitative and sensitive analyses of drugs and peptides
in routine research environments.3 Interestingly, most
LC-HRMS analyses are performed in HR full scan
acquisition which allows an overview of all ions such as
steroid metabolites.
The work presented here is to analyze quantitatively
eight steroids using an affordable HRMS instrument, the
Thermo Scientific™ Q Exactive™ Focus MS, for clinical
research purposes.
A robust and rapid LC-MS method for the determination
of steroids in plasma samples using an affordable HRMS
instrument has been presented and is suitable for routine
research analyses. Results acquired using this method
demonstrate that the Q Exactive Focus MS exhibits
accuracy, precision, and sensitivity that is comparable to
triple quadrupole MS instruments. Various steroid isomers
can be detected in full scan but also in MS/MS acquisition
showing that there is a need for an efficient
chromatography. In addition, with sensitive and selective
full scan data, the Q Exactive Focus HRMS can be used
for more in-depth investigations when requested by
researchers or biochemists.
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