方案摘要
方案下载| 应用领域 | 制药/生物制药 |
| 检测样本 | 化药新药研发 |
| 检测项目 | 化合物发现>先导化合物 |
| 参考标准 | 微波多肽合成 |
CEM专利的环形电磁场结构设计，使氨基酸构成的卷曲肽链充分展开，进行彻 底的去保护、耦合和裂解，反应变得极其快速，完全，高纯。Liberty BLUE比传统方法提高了近20倍；标准的10肽 ACP 序列合成纯度竟达到 98%，使得许多合成反应甚至可免去纯化步骤；可以完成传统方法不可能实现的困难合成。实现更多更长的氨基酸耦合，防止长链多肽聚合，消除双重耦合和外消旋现象，同时降低树脂的要求。BLUE 开辟了多肽合成的新纪元，使生物化学和蛋白质研究具备了空前的多肽合成的能力，是以前传统合成方法根本无法想象的。市场上没有任何一台多肽合成仪能与 BLUE相媲美。
清华大学刘磊教授团队用CEM LIBERTY BLUE全自动微波多肽合成仪合成了长达228个氨基酸的多肽，是目前世界上最大的合成结晶纯蛋白，做出如此世界级的突破性成绩！祝贺他们！
文章发表在最新一期的美国化学学会杂志上，摘要如下：
Quasi-Racemic X-ray Structures of K27-Linked Ubiquitin Chains Prepared by Total Chemical Synthesis
Man Pan†, Shuai Gao†, Yong Zheng†, Xiaodan Tan†, Huan Lan†, Xianglong Tan†, Demeng Sun†, Lining Lu†, Tian Wang†, Qingyun Zheng†, Yichao Huang†, Jiawei Wang‡ and Lei Liu*†
*lliu@mail.tsinghua.edu.cn
†Tsinghua-Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China
‡State Key Laboratory of Biomembrane and Membrane Biotechnology, Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China
Journal of the American Chemical Society
Publication Date (Web): June 6, 2016
DOI: 10.1021/jacs.6b04031
Quasi-racemic crystallography has been used to determine the X-ray structures of K27-linked ubiquitin (Ub) chains prepared through total chemical synthesis. Crystal structures of K27-linked di- and tri-ubiquitins reveal that the isopeptide linkages are confined in a unique buried conformation, which provides the molecular basis for the distinctive function of K27 linkage compared to the other seven Ub chains. K27-linked di- and triUb were found to adopt different structural conformations in the crystals, one being symmetric whereas the other triangular. Furthermore, bioactivity experiments showed that the ovarian tumor family de-ubiquitinase 2 significantly favors K27-linked triUb than K27-linked diUb. K27-linked triUb represents the so-far largest chemically synthesized protein (228 amino acids) that has been crystallized to afford a high-resolution X-ray structure.
【CEM】酸奶中的蛋白质
【CEM】电子设备组件样品制备用于IEC 62321-7-2:2017方法六价铬含量分析
【eralytics】结合蒸气压与密度的测量
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