资料摘要
资料下载The study deals with the adhesion and proliferation of bovine retina pericytes onto surfaces of poly(hydroxymethylsiloxane) (PHMS) modified either by cold plasma or by low-energy ion beams. The surface treatment was able to convert the original polymer matrix into SiO2-like phases for O2-plasma or ion-mixed SiCxOy(Hz) phases for ion irradiation, respectively, with different modification levels of the surface free energy (SFE) and related surface wettability. Pericytes exhibited a negligible adhesion and proliferation onto untreated PHMS, an enhanced adhesion but not proliferation on plasma-treated PHMS, and great adhesion and proliferation to full confluence on ion-irradiated PHMS, as measured by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), quartz crystal microbalance, and optical microscopy. On the other hand, the adhesion and proliferation of GP8.39 endothelial cells (EC), which are strongly associated with pericytes in microvasculature, were very scarce onto both untreated and surface-modified PHMS. The surface-selective pericytal response was related to changes of physicochemical properties of PHMS film, from hydrophobic/neutral towards hydrophilic/negatively charged polymer layers, as well as to short- and long-time events of cell–surface interaction. We propose that surface properties can mediate and modulate cell–polymer matrix adhesion through the establishment of stereospecific chemical interactions and/or electrostatic repulsion, which can also explain the different behavior of pericytes compared to EC.
CO150 CO2培养箱——细胞培养“兵工厂”
简介:Thermofisher Labserv CO150 CO2培养箱,易于操作和维护,为您细胞培养提供了良好的生存环境,并且添加了有效的湿热灭菌功能,是您细胞安心培养的“兵工厂”。
真空冷冻干燥机ALPHA 1-2 LDplus的典型实验应用简介
简介:熟悉ALPHA 1-2 LDplus冻干机的使用者都了解,其广泛应用于植物提取物、生物样品、药物制剂研究中的冻干实验。但除了这些典型的应用,仍有一些其他典型实验,也是可以用到ALPHA 1-2 LDplus的。那我们就来看看,还有哪些是我们未知或不熟悉的典型应用:
浓缩仪浓缩过程中样品温度与真空度、加热温度
简介:德国Christ公司是专业生产真空离心浓缩仪的厂家,有着60多年的历史。就其浓缩仪中样品温度的变化,我们进行了详细的数据记录(图1、图2所示),从图1我们发现,当真空值设为7mbar,腔体加热温度设为79℃时,离心腔在61~79℃逐渐升温,对应的转子温度是51.1~71.7℃逐渐升温,而样品温度在14.7~18.2度之间。
常规实验中离心容量及转速一览表
简介:离心机是实验室最常用的仪器设备,在任何实验中,离心时的容量需求以及转速表现是使用者最关心的两个方面,那我们就来梳理下常规实验中所需的离心容量及转速情况:
不一样的紫外能量监控系统
简介:TOC(总有机碳)是水中有机物质的总含量,以有机物中的主要元素---碳的量来表示。随着国家对水质污染的进一步重视,TOC监测作为评价水体中有机物污染程度的一项重要参考指标,已越来越受到重视。而在实验室中,随着生化分析方法对低水平有机物灵敏度的提高,监测产水中TOC含量的重要性亦日趋凸显。例如用于高效液相色谱仪、气相色谱质谱联用仪等对有机物敏感的应用,水中不含有机物这一点至关重要。
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