多功能单细胞显微操作系统- FluidFM OMNIUM

多功能单细胞显微操作系统- FluidFM OMNIUM

一款全自动高精度单细胞操纵平台

设备型号已正式由FluidFM BOT更新为FluidFM OMNIUM

  单细胞提取

——FluidFM技术是微量、低创、的活细胞原位提取方案

直接细胞自然生长环境中提取单个细胞的内容物，同时不影响细胞活力。该程序是破坏性低的，实验证明，可以在不同时间点对同一单个细胞进行细胞活组织检查。

  单细胞分离

——FluidFM技术是进行无损细胞分离、分选、构建细胞系的理想工具

●  直观、简单、温和、自动化；

●  的挑选和放置细胞：选择你想要的细胞，并把它放在您需要的位置

●  分离通量：约50个细胞/小时。

可以将分离后的细胞放置任何您需要的位置，也可以按照不同的形状位置去放置细胞。

  单细胞注射

——FluidFM技术是将多种不同类型物质注射入单细胞的佳选择

●  无损注入的将不同类型的物质准确注入到细胞质或者细胞核。
●  可定量的fL别注射。
●  注射后细胞存活率>95%。

●  每小时可注射>100个细胞。

注射细胞种类：

贴壁、悬浮细胞均可，对于注射细胞的种类，FluidFM并没有限制，而且，心肌细胞这种注射难度高的细胞也能够胜任。

注射任何可溶性化合物：

Lucifer Yellow、UHRF1-BFP proteins、Small RNAs、Plasmids、CRISPR/Cas9 RNP complexes、Antibodies等。

  单细胞力谱

——FluidFM技术在单细胞水平上提供高质量的力学数据

●  细胞水平力学测定通量高、直接抓取或者压细胞即可。

●  适用细胞种类：悬浮细胞、贴壁细胞均可测量

●  力学范围：nN到μN别的细胞水平的力

●  操作简单：区别于AFM细胞力学测量，具有简便、直接的优势。

测试数据

肝细胞的微量注射

HeLa细胞的微量提取

CHO细胞的单细胞分离

纳米光刻DAPI染料

发表文章

单细胞注射：

1. O.Guillaume-Gentil, E.Potthoff, D.Ossola, et al. Force-controlled fluidic injection into single cell nuclei.(2013)Small,9(11),1904?1907. doi:10.1002/ smll.201202276A.

2. Meister, M. Gabi, P.Behr, et al. FluidFM: Combining atomic force microscopy and nanofluidics in a universal liquid delivery system for single cell applications and beyond.(2009) Nano Letters, 9(6), 2501?2507. doi:10.1021/nl901384x

单细胞提取：

1. O. Guillaume-Gentil, T. Rey, P. Kiefer, A.J. Ibá?ez, R. Steinhoff, R. Br?nnimann, L. Dorwling-Carter, H. Zambelli, R. Zenobi & J.A. Vorholt. Single-Cell Mass Spectrometry of Metabolites Extracted from Live Cells by Fluidic Force Microscopy. (May 2017) Anal Chem., 89(9), 5017-5023. doi:10.1021/acs.analchem.7b00367

2. O. Guillaume-Gentil, R.V. Grindberg, R. Kooger, L. Dorwling-Carter, V. Martinez, D. Ossola, M. Pilhofer, T. Zambelli & J.A. Vorholt. Tunable Single-Cell Extraction for Molecular Analyses. (Jul 2016) Cell, 166(2), 506-516. doi: 10.1016/j. cell.2016.06.025.

单细胞分离：

1. O. Guillaume-Gentil, T. Zambelli & J.A. Vorholt.Isolation of single mammalian cells from adherent cultures by fluidic force microscopy. (2014) Lab on a chip, 14(2), 402-414. doi:10.1039/c3lc51174j

2. P. Stiefel, T. Zambelli & J.A. Vorholt. Isolation of optically targeted single bacteria by application of fluidic force microscopy to aerobic anoxygenic phototrophs from the phyllosphere. (2013) Applied and Environmental Microbiology, 79(16), 4895-4905. doi:10.1128/AEM.01087-13P.

3. D?rig, P. Stiefel, P. Behr, et al. Force-controlled spatial manipulation of viable mammalian cells and micro-organisms by means of FluidFM technology.(2010) Applied Physics Letters, 97(2), 023701 1-3. doi:10.1063/1.3462979

新发表：

2021

1. M. Mathelié-Guinlet, F. Viela, J. Dehullu, S. Filimova, J.M. Rauceo, P.N. Lipke & Y.F. Dufrêne. Single-cell fluidic force microscopy reveals stress-dependent molecular interactions in yeast mating. (2021) Commun Biol. doi: 10.1038/s42003-020-01498-9
AFM Series: Adhesion of single cells

2020

1. A.G. Nagy, A. Bonyár, I. Székács & R. Horvath. Analysis of single-cell force-spectroscopy data of Vero cells recorded by FluidFM BOT. (2020) IEEE 26th International Symposium for Design and Technology in Electronic Packaging (SIITME). doi: 10.1109/SIITME50350.2020.9292265, BIO Series: Adhesion of single cells

2. I. Demir, J. Blockx, E. Dague, P. Guiraud, W. Thielmans, K. Muylaert & C. Formosa-Dague. Nanoscale Evidence Unravels Microalgae Flocculation Mechanism Induced by Chitosan. (2020) ACS Applied Biomaterials. doi: 10.1021/acsabm.0c007722. AFM Series: Adhesion of single cells

3. P. Saha, T. Duanis-Assaf & M. Reches. Fundamentals and Applications of FluidFM Technology in Single-Cell Studies. (2020) Advanced Materials Interfaces. doi: 10.1002/admi.20001115. AFM Series: REVIEW

4. T. Schlotter, S. Weaver, C. Forró, D. Momotenko, J. Voros, T. Zambelli & M. Aramesh. Force-Controlled formation of dynamic nanopores for single-biomolecule sensing and single-cell secretomics. (2020) ACS Nano. doi: 10.1021/acs.nano.0c04281. AFM Series: SICM, other

5. L. Hofherr, C. Müller-Renno, C. Ziegler. FluidFM as a tool to study adhesion forces of bacteria - Optimization of parameters and comparison to conventional bacterial probe Scanning Force Spectroscopy. (2020). PLOS ONE. doi: 10.1371/journal.pone.0227395. AFM Series: Adhesion of single bacteria

6. T. Schlotter, S. Weaver, T. Zambelli, J. Voros & M. Aramesh. Force-controlled nanopores for single cell measurements using micro-channelled AFM Cantilevers. (2020). Biophysical Journal. doi: 10.1016/j.bpj.2019.11.1066. AFM Series: Other

7. J. Zhang, H. Yu, B. Harris, Y. Zheng, U. Celik, L. Na, R. Faller, X. Chen, D. R. Haudenschild, G. Liu. New Means to Control Molecular Assembly (2020) ACS Publications. doi.org/10.1021/acs.jpcc.9b11377. BIO Series: Nanolithography

8. P. Wysotzki, A. Sancho, J. Gimsa, J. Groll.  A comparative analysis of detachment forces and energies in initial and mature cell-material interaction (2020) Science Direct. doi.org/10.1016/j.colsurfb.2020.110894. AFM Series: Single Force Spectroscopy

9. M. Sztilkovics, T. Gerecsei, B. Peter, A. Saftics, S. Kurunczi, I. Szekacs, B. Szabo & R. Horvath. Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy. (2020) Scientific Reports. doi: 10.1038/s41598-019-56898-7. BIO Series: Adhesion of single cells

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