| 供货周期： | 一周 |
| 品牌： | 安捷伦 |
| 货号： | 212216 |
相关产品
Agilent TC-C18 4.6 x 150 x 5u
Agilent HC-C8 4.6 x 150 x 5u
Agilent HC-C18 4.6 x 150 x 5u
Agilent TC-C18
Agilent HC-C18
Agilent HC-C8
镀金参比帽，镜面反射，用于 Agilent 4300 手持式 FTIR
参比帽，漫反射，粗糙，用于 Agilent 4300 手持式 FTIR
参比帽，镜面反射，不锈钢，用于 Agilent 4300 手持式 FTIR
镀金参比帽，粗糙，用于 Agilent 4300 手持式 FTIR
参比样品，聚苯乙烯，用于 Agilent 4300 手持式 FTIR
背投反射镜，氘化硫酸三甘氨酸 (DTGS)，用于 Agilent 4300 手持式 FTIR
背投反射镜，碲镉汞 (MCT)，用于 Agilent 4300 手持式 FTIR
SoMo 655 PDA 升级替代包，用于 Agilent 4100/4200/4500 FTIR
4100 ExoScan FTIR Polystyrene frequency reference cap for use with external reflectance sample interface.
关注
拨打电话
留言咨询
![](https://www.agilent.com/common/requestQuote.jsp?source=contactus>联系我们</a> </li></ul>
</div>
</header>
</section>
<footer>
<a class="pull-right" href="#"> 返回页首<i class="fa fa-chevron-circle-up"></i></a>
</footer>
</section>
<!-- End of features section from the Consumables Product Asset -->
<!--templateName->/SpecificationsSectionPageletTemplate -->
<!--templateName->/TechnologySectionPageletTemplate -->
<!--templateName->/SideBySideSectionPageletTemplate -->
<script type="text/javascript">
var sections = {"section1":{"template":"SideBySideImageTemplate","templateId":"1405222579069_0","id":"section_1405222579069","name":"pBC Phagemid Vectors","nameId":"1405222579069_1406546460262","assettype":"Section_C"}};
</script>
<script type="text/javascript">
var howItWorksSection = {"section":{"data":{"attributes_list":"id,home_section_title,section_cta_title,analytics_section_title,section_cta_url,section_links,section_sub_text,component_headline,component_desc","id":"1405222579069","idId":"1405222579069_0","home_section_title":"pBC Phagemid Vectors","home_section_titleId":"1405222579069_1406546460262","analytics_section_title":"NoAnalytics","analytics_section_titleId":"1405222579069_1405257770331","assettype":"Section_C","slots":[{"ckeditorAttributes":"slot_basic_desc","attributes_list":"slot_title,slot_basic_desc,slot_digital_image,image_allign,grey_background,slot_digital_imageZoom,slot_video,cta_label,slot_url,display,id,page-url,label","slot_title":"Promoters and polylinkers","slot_titleId":"1405222579203_1406546459576","slot_basic_desc":"<table border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"402\">\r\n\t<colgroup>\r\n\t\t<col width=\"402\" />\r\n\t</colgroup>\r\n\t<tbody>\r\n\t\t<tr height=\"240\">\r\n\t\t\t<td class=\"xl65\" height=\"240\" style=\"height:180.0pt;width:302pt\" width=\"402\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tThe Phagemid has a polylinker with 21 unique restriction enzyme recognition sites. The T7 and T3 RNA polymerase promoters can be used to synthesize RNA <font class=\"font5\">in vitro</font><font class=\"font0\">. The promoter used to initiate transcription determines which strand of the insert cloned into the polylinker will be transcribed.<br />\r\n\t\t\t\t\t<br />\r\n\t\t\t\t\tpBC (+) and (–) are available with two polylinker orientations: KS and SK. For the KS orientation, the Kpn I restriction site is nearest the lacZ promoter and the Sac I restriction site is farthest from the lacZ promoter. For the SK orientation, it is vice versa.</font></p>\r\n\t\t\t\t<p>\r\n\t\t\t\t\t </p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t</tbody>\r\n</table>\r\n<p>\r\n\t </p>\r\n","slot_basic_descId":"1405222579203_1457965536647","slot_digital_imageZoom":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_image":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_imageId":"1405222579203_1457965621962","image_allign":"Left","image_allignId":"1405222579203_1457965561153","grey_background":"White","grey_backgroundId":"1405222579203_1457965561160","display":"/SideBySideImageTemplate","id":"1405222579203","assettype":"Slots_C"},{"ckeditorAttributes":"slot_basic_desc","attributes_list":"slot_title,slot_basic_desc,slot_digital_image,image_allign,grey_background,slot_digital_imageZoom,slot_video,cta_label,slot_url,display,id,page-url,label","slot_title":"A total of 131 amino acids","slot_titleId":"1405222579549_1406546459576","slot_basic_desc":"<table border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"350\">\r\n\t<colgroup>\r\n\t\t<col width=\"350\" />\r\n\t</colgroup>\r\n\t<tbody>\r\n\t\t<tr height=\"240\">\r\n\t\t\t<td class=\"xl65\" height=\"240\" style=\"height:180.0pt;width:263pt\" width=\"350\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tThe polylinker and T7 and T3 RNA polymerase promotor sequences are present in the N-terminal portion of a lacZ gene fragment. There are 36 amino acids from the MET sequence to the EcoR I site in the pBC plasmid. There are a total of 131 amino acids, but this is interrupted by the large polylinker. pBC phagemids that have no inserts in the polylinker will grow as blue colonies on the appropriate strains of bacteria. pBC phagemids that have inserts will grow as white colonies on the same strain, because the inserts disrupt the coding region of the lacZ gene fragment.</p>\r\n\t\t\t\t<p>\r\n\t\t\t\t\t </p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t</tbody>\r\n</table>\r\n<p>\r\n\t </p>\r\n","slot_basic_descId":"1405222579549_1457965536647","slot_digital_imageZoom":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_image":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_imageId":"1405222579549_1457965621962","image_allign":"Right","image_allignId":"1405222579549_1457965561153","grey_background":"White","grey_backgroundId":"1405222579549_1457965561160","display":"/SideBySideImageTemplate","id":"1405222579549","assettype":"Slots_C"},{"ckeditorAttributes":"slot_basic_desc","attributes_list":"slot_title,slot_basic_desc,slot_digital_image,image_allign,grey_background,slot_digital_imageZoom,slot_video,cta_label,slot_url,display,id,page-url,label","slot_title":"Available with f1 origin in either (+) or (-) orientation","slot_titleId":"1405222579951_1406546459576","slot_basic_desc":"<table border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"341\">\r\n\t<colgroup>\r\n\t\t<col width=\"341\" />\r\n\t</colgroup>\r\n\t<tbody>\r\n\t\t<tr height=\"240\">\r\n\t\t\t<td class=\"xl65\" height=\"240\" style=\"height:180.0pt;width:256pt\" width=\"341\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tThe BssH II sites are rare six-base cutters that allow the insert plus the T phage RNA promotors to be excised and used for gene mapping.<br />\r\n\t\t\t\t\t<br />\r\n\t\t\t\t\tpBC phagemids can be rescued as single-stranded DNA and contain a 454-nucleotide filamentous f1 phage intergenic region. The (+) and (–) orientations of the f1 intergenic region allow the rescue of sense or antisense ssDNA by a helper phage. This ssDNA can be used for dideoxynucleotide sequencing or site-specific mutagenesis.</p>\r\n\t\t\t\t<p>\r\n\t\t\t\t\t </p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t</tbody>\r\n</table>\r\n<p>\r\n\t </p>\r\n","slot_basic_descId":"1405222579951_1457965536647","slot_digital_imageZoom":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_image":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_imageId":"1405222579951_1457965621962","image_allign":"Left","image_allignId":"1405222579951_1457965561153","grey_background":"White","grey_backgroundId":"1405222579951_1457965561160","display":"/SideBySideImageTemplate","id":"1405222579951","assettype":"Slots_C"}]}}};
</script>
<section class="item-collection clear pdpSections active operation-block-section">
<header class="col-full operation-block-header">
<div id="howitworks" class="heading" role="heading" aria-level="3">工作原理</div>
</header>
<section class="item col-full operation-block" data-section="pdp:operation">
<div class="media-body" >
<how-it-works></how-it-works>
<script>
var x=document.querySelector(){kind=link}