全自动液液萃取——VERSA1100

2020/04/14   下载量： 2 次

方案摘要

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应用领域 食品/农产品
检测样本 畜禽肉及副产品
检测项目
参考标准 VERSA 1100自动油脂提取工作站也与非氯化溶剂系统（如MTBE和BUME方法）兼容

液-液萃取（LLE）法是油脂组学研究中常用的质谱样品制备方法。VERSA 1100自动液–液萃取工作站已配置为使用氯仿：甲醇（Bligh and Dyer或Folch）方法从生物样品中提取脂质，以减少人为误差并提高效率。

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全自动液液萃取——VERSA1100

Liquid-liquid extraction (LLE) method is commonly used for mass spectrometry sample preparation in lipidomics research. The VERSA 1100 Automated Liquid-Liquid Extraction Workstation has been configured to extract lipids from biological samples using the chloroform:methanol (Bligh and Dyer or Folch) method to reduce human error and increase efficiency.

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Below are the data generated from extracting lipids from chicken liver samples. In brief, samples are extracted in 15x85mm glass tubes in 24 sample/batch. This automated procedure includes adding solvents, mixing, phase separation, drying, reconstitution, adding internal standard and sample transfer. Compared to manual chloroform:methanol lipid extraction experiment, the automated extraction efficiency was found to be 94.3% and the coefficient of variation (CV) for the extraction of top 200 lipids from chicken liver using VERSA 1100 was 2-fold lower than that obtained with manual extraction. The VERSA 1100 Automated Lipid Extraction workstation is also compatible with non-chlorinated solvent systems such as MTBE and BUME methods.



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加拿大欧罗拉生物科技有限公司
金牌会员 丨 第17年
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