海白菜(石莼)总脂肪和蛋白质的测定

2024/03/21   下载量: 0

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应用领域 食品/农产品
检测样本 其他水产制品
检测项目 营养成分>脂肪, 蛋白质
参考标准 GB 5009.6 食品中脂肪的测定

不同收获时间海白菜生化成分及其与颜色的关系The biochemical composition and its relation to color of Ulva spp. upon harvest time 使用格哈特DUMATHERM杜马斯定氮仪检测蛋白质含量,蛋白质系数为5,每毫克样品1.4 毫克氧气,氧气流量200毫升每分钟。 Total nitrogen was analyzed by Dumas combustion (DUMATHERM,Gerhart Analytical Systems, Germany) using 1.4 mg O2 mg-1 sample and an O2 fowrate of 200 mL min-1. The CP content was calculated using a N-to-protein conversion factor of 5. 总脂肪使用格哈特Hydrotherm全自动超级酸水解打破样品的脂蛋白键后再用格哈特Soxtherm全自动快速索氏提取脂肪。 The lipid content of the biomasses was quantifed in triplicates by digesting one gram of sample with boiling hydrochloric acid to break the lipo-protein bonds using a Hydrotherm (Gerhardt Analytical systems, Germany). The resulting solution was collected in a flter, dried, and the lipid was subsequently extracted with petroleum ether using a Soxtherm (Gerhardt Analytical systems, Germany).

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不同收获时间海白菜生化成分及其与颜色的关系The biochemical composition and its relation to color of Ulva spp. upon harvest time


使用格哈特DUMATHERM杜马斯定氮仪检测蛋白质含量,蛋白质系数为5,每毫克样品1.4 毫克氧气,氧气流量200毫升每分钟。

Total nitrogen was analyzed by Dumas combustion (DUMATHERM,Gerhart Analytical Systems, Germany) using 1.4 mg O2 mg-1 sample and an O2 fowrate of 200 mL min-1. The CP content was calculated using a N-to-protein conversion factor of 5.CG_Realyzer_product photo_06.jpg

总脂肪使用格哈特Hydrotherm全自动超级酸水解打破样品的脂蛋白键后再用格哈特Soxtherm全自动快速索氏提取脂肪。

The lipid content of the biomasses was quantifed in triplicates by digesting one gram of sample with boiling hydrochloric acid to break the lipo-protein bonds using a Hydrotherm (Gerhardt Analytical systems, Germany). The resulting solution was collected in a flter, dried, and the lipid was subsequently extracted with petroleum ether using a Soxtherm (Gerhardt Analytical systems, Germany).

Photo_HYDROTHERM + SOXTHERM, 1.jpg

上一篇 使用格哈特系列仪器进行添加植物成分的火鸡大腿肉丸中蛋白质、脂肪、膳食纤维的测定
下一篇 油茶壳木质纤维素(纤维素、半纤维素和木质素)的测定

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