供货周期: | 现货 |
品牌: | 赛默飞 |
货号: | 85087085089 |
Dionex ERD 500 电解再生脱盐器
离子色谱分析 (IC) 期间,从高 pH 值洗脱液中轻松收集低聚糖馏分,并自动降低 pH 值和去除盐分。Thermo Scientific Dionex ERD 500 电解再生脱盐器在线从碱性乙酸钠废液中去除钠离子 (Na+),例如高 pH 值阴离子交换色谱分析及脉冲安培检测 (HPAE-PAD) 应用后。
在低聚糖分离中自动去除盐并减低 pH 值
Dionex ERD 500 电解再生脱盐器通过将钠离子替换为水合氢离子来降低样本 pH 值并去除超过 99% 的钠离子,从而将氢氧化钠和乙酸钠洗脱液转换成水和乙酸。挥发性乙酸使得在无需透析的情况下获得冻干馏分成为可能,让经过纯化的糖类样品可用于进一步操作。
将废液的 pH 值从 ~13 降为 2-6
冻干收集的馏分时,无需透析,节省人力
从含高达0.35 M Na+ 的洗脱液中去除超过 99% 的钠离子
最大程度分散从检测器单元中排出的样品,准确收集馏分
使用电解和三氟丙基酸 (TFA) 再生剂提供持续的脱盐器再生
High-pH HPAE-PAD has become the method of choice for oligosaccharide purification because it permits very high resolution of neutral and charged oligosaccharide isomers. Subsequent characterization of purified oligosaccharides often requires removal of eluent salts. Manual desalting methods are available; the Thermo Scientific™ Dionex™ ERD™ 500 Electrolytcally Regenerated Desalter provides an easier, automated method.
Reduces the pH of the effluent from pH ~13 to between pH 2–6
Supports lyophilization of collected fractions without dialysis
Provides minimal dispersion of the sample exiting the detector cell
Low dispersion for accurate fraction collection
>99% sodium ions removed from eluents that contain up to 0.35 M sodium ions
The Dionex ERD 500 desalter exchanges sodium ions in the eluent for hydronium ions. This process converts sodium hydroxide and sodium acetate eluents to water and acetic acid, lowering sample pH after samples leave the detector cell. Because acetic acid is volatile, fractions can then be lyophilized without dialysis, leaving the purified carbohydrate sample ready for further manipulation.
The Dionex ERD 500 desalter reliably desalts up to 0.35 M Na at an eluent flow of 1.0 mL/min. Oligosaccharides eluting in up to 0.35 M Na, collected after on-line desalting and evaporated in a centrifugal vacuum evaporator, exhibit residual [Na+] below 200 μM after resuspension to original volume in deionized water. This represents a desalting efficiency of >99.9%.
订货信息:
Ordering Information | |
---|---|
ERD 500 Electrolytically Regenerated Desalter (4 mm) | 085087 |
ERD 500 Electrolytically Regenerated Desalter (2 mm) | 085089 |
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