PRINCIPLE OF THE METHOD

2014-04-17 14:46  下载量：1

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PRINCIPLE OF THE METHOD The COX-1 kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known COX-1 concentrations and unknowns are pipetted into these wells. During the first incubation, the COX-1 antigen and a biotinylated monoclonal antibody specific for COX-1 are simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of COX-1 present in the samples.

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