Goat anti- Guinea Pig Interleukin-4

2014-04-21 10:00 下载量：0

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Goat anti- Guinea Pig Interleukin-4 Collect Sample – Tissue homogenate Storage: 2 - 8 °C Package size: 96 determinations PRINCIPLE OF THE METHOD The IL-4 kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known IL-4 concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-4 antigen and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-4 present in the samples.

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上海信裕生物科技有限公司

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