Rat DNMT

2014-04-21 10:59  下载量:0

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Rat DNMT FOR RESEARCH USE ONLY Assay range:7 pg/ml - 260pg/ml 96 determinations Purpose This kit allows for the determination of DNMT concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat DNMT level in the sample,use Purified Rat DNMT antibody to coat microtiter plate wells, make solid-phase antibody, then add DNMT to wells, Combined DNMT antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat DNMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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