资料摘要
资料下载Rat PCNA FOR RESEARCH USE ONLY Assay range：0.1ng/ml -4ng/ml 96 determinations Purpose This kit allows for the determination of PCNA concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat PCNA level in the sample, use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add PCNA to wells, Combined antigen which With HRP labeled, become antigen – antibody - enzyme-antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat PCNA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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