资料摘要
资料下载Rat Prostaglandin E2(PGE2) ELISA Kit FOR RESEARCH USE ONLY Assay range：15ng/L - 480ng/L 96 determinations Purpose This kit allows for the determination of PGE2 concentration in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat PGE2 level in the sample，use Purified Rat PGE2 antibody to coat microtiter plate wells, make solid-phase antibody, then add PGE2 to wells, Combined PGE2 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat PGE2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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