Rat Dihydrotestosterone(DHT)ELISA Kit

2014-04-21 11:33  下载量:0

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Rat Dihydrotestosterone(DHT)ELISA Kit FOR RESEARCH USE ONLY Assay range:1.5nmol/L - 48nmol/L 96 determinations Purpose This kit allows for the determination of DHT concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat DHT level in the sample, use Purified Rat DHT antibody to coat microtiter plate wells, make solid-phase antibody, then add DHT to wells, Combined DHT antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat DHT in the samples

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