资料摘要
资料下载Rat nitric oxide (NO) FOR RESEARCH USE ONLY Assay range：1μmol/L -40μmol/L 96 determinations Purpose This kit allows for the determination of NO concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat NO level in the sample, use Purified Rat NO antibody to coat microtiter plate wells, make solid-phase antibody, then add NO to wells, Combined NO antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat NO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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