资料摘要
资料下载Rat interleukin 18(IL-18) FOR RESEARCH USE ONLY Assay range：3ng/L -160 ng/L 96 determinations Purpose This kit allows for the determination of IL-18 concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat IL-18 level in the sample，use Purified Rat IL-18 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-18 to wells, Combined IL-18 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat IL-18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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