Rat LPS

2014-04-21 12:34  下载量:0

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Rat LPS FOR RESEARCH USE ONLY Assay range:0.03Eu/L – 0.8Eu/L 96 determinations Purpose This kit allows for the determination of LPS concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat LPS level in the sample, use Purified Rat LPS antibody to coat microtiter plate wells, make solid-phase antibody, then add LPS to wells, Combined LPS antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat LPS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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