资料摘要
资料下载Goat Anti-Rat prolyl hydroxylase Storage: 2 - 8 °C Package size: 96 determinations PRINCIPLE OF THE METHOD The PHD kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known PHD concentrations and unknowns are pipetted into these wells. During the first incubation, the PHD antigen and a biotinylated monoclonal antibody specific for PHD are simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PHD present in the samples.
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