Rat bone alkaline phosphatase (BALP)

2014-04-21 15:58 下载量：0

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Rat bone alkaline phosphatase (BALP) FOR RESEARCH USE ONLY Assay range：0.5μg/L -16μg/L 96 determinations Purpose This kit allows for the determination of BALP concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat BALP level in the sample，use Purified Rat BALP antibody to coat microtiter plate wells, make solid-phase antibody, then add BALP to wells, Combined BALP antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat BALP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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上海信裕生物科技有限公司

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