Rat β-CG

2014-04-21 17:19 下载量：0

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Rat β-CG FOR RESEARCH USE ONLY Assay range：1IU/L - 32IU/L 96 determinations Purpose This kit allows for the determination of β-CG concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat β-CG level in the sample，use Purified Rat β-CG antibody to coat microtiter plate wells, make solid-phase antibody, then add β-CG to wells, Combined β-CG antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat β-CG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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上海信裕生物科技有限公司

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