Human Kim-1

2014-04-21 17:21 下载量：0

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Human Kim-1 FOR RESEARCH USE ONLY Assay range：5ng/L - 160ng/L 96 determinations Purpose This kit allows for the determination of Kim-1 concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human Kim-1 level in the sample，use Purified Human Kim-1 antibody to coat microtiter plate wells, make solid-phase antibody, then add Kim-1 to wells, Combined Kim-1 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Kim-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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上海信裕生物科技有限公司

银牌会员 | 第11年

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