Microcystins LR(MC-LR)

2014-04-22 09:27  下载量:1

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Microcystins LR(MC-LR) FOR RESEARCH USE ONLY Assay range:7 pg/mL - 300pg/mL 96 determinations Purpose This kit allows for the determination of MC-LR concentrations in Water samples and other related samples. Principle of the assay The kit assay MC-LR level in the sample, use Purified MC-LR antibody to coat microtiter plate wells, make solid-phase antibody, then add MC-LR to wells, Combined MC-LR antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MC-LR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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