Carp vitellogenin (VTG)

2014-04-22 10:00 下载量：0

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Carp vitellogenin (VTG) FOR RESEARCH USE ONLY Assay range：30μg/L -850μg/L 96 determinations Purpose This kit allows for the determination of VTG concentrations in zebra fish serum, cell culture supernates and other biological fluids Principle of the assay The kit assay zebra fish VTG level in the sample，use Purified zebra fish VTG antibody to coat microtiter plate wells, make solid-phase antibody, then add VTG to wells, Combined VTG antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VTG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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上海信裕生物科技有限公司

银牌会员 | 第11年

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