heep TNF-α

2014-04-22 10:54  下载量:0

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heep TNF-α FOR RESEARCH USE ONLY Assay range:25 ng/L-800 ng/L 96 determinations Purpose This kit allows for the determination of TNF-αconcentrations in Sheep serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Sheep TNF-α level in the sample,use Purified Sheep TNF-α antibody to coat microtiter plate wells, make solid-phase antibody, then add TNF-α to wells, Combined TNF-α antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Sheep TNF-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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