fish estradiol （E2）

2014-04-22 11:10 下载量：0

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fish estradiol （E2） FOR RESEARCH USE ONLY Assay range：2.5 ng/L - 80ng/L 96 determinations Purpose This kit allows for the determination of E2 concentrations in zebra fish serum, cell culture supernates and other biological fluids Principle of the assay The kit assay zebra fish E2 level in the sample，use Purified zebra fish E2 antibody to coat microtiter plate wells, make solid-phase antibody, then add E2 to wells, Combined E2 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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上海信裕生物科技有限公司

银牌会员 | 第11年

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