资料摘要
资料下载Fish Follicle-stimulating hormone (FSH) FOR RESEARCH USE ONLY Assay range：1U/L -32U/L 96 determinations Purpose This kit allows for the determination of FSH concentrations in Fish serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Fish FSH level in the sample，use Purified Fish FSH antibody to coat microtiter plate wells, make solid-phase antibody, then add FSH to wells, Combined FSH antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Fish FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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