Human interleukin-17(IL-17) ELISA kit

2015-10-09 10:10  下载量:2

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Human interleukin-17(IL-17) FOR RESEARCH USE ONLY Assay range:0.7 ng/L -20 ng/L 96 determinations Purpose This kit allows for the determination of IL-17 concentrations in Human serum. Principle of the assay The kit assay Human IL-17 level in the sample, use Purified Human IL-17 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-17 to wells, Combined IL-17 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human IL-17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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