赤霉素(GA)检测试剂盒

赤霉素(GA)检测试剂盒

适用生物     General，通用    

赤霉素(GA)检测试剂盒   

检测范围     123.46-10000ng/mL     灵敏度     42.9ng/mL    

样本类型     Tissue or cell culture supernates.    

实验时长     2.5h     实验方法     竞争抑制法     赤霉素(GA)检测试剂盒站    

规格     96T    

ELISA Kit for Gibberellic Acid (GA)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species     General    

赤霉素(GA)检测试剂盒 

Sample type     Tissue or cell culture supernates.    

Format     96-well strip plate    

Assay length     2.5 hours    

Detection range     123.46-10000ng/mL The standard curve concentrations used for the ELISA’s were 10000ng/mL, 3333.33ng/mL, 1111.11ng/mL, 370.37ng/mL, 123.46ng/mL    

Sensitivity     The minimum detectable赤霉素(GA)检测试剂盒 dose of this kit is typically less than 42.9ng/mL.    

Specificity

This assay has high sensitivity and excellent specificity for detection of Gibberellic Acid (GA).
No significant cross-reactivity or interference between Gibberellic Acid (GA) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gibberellic Acid (GA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gibberellic Acid (GA) were tested 赤霉素(GA)检测试剂盒on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the赤霉素(GA)检测试剂盒 performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents     Quantity     Reagents     Quantity    

Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    

Standard     2     Standard Diluent     1×20mL    

Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    

Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    

TMB Substrate     1×9mL     Stop Solution     1×6mL    

Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50μL standard or sample to each well.
    And then add 50μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90μL Substrate Solution. 赤霉素(GA)检测试剂盒Incubate 15-25 minutes at 37oC;
7. Add 50μL Stop Solution. Read at 450 nm immediately.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gibberellic Acid (GA) has been pre-coated onto a microplate. A competitive inhibition reaction 赤霉素(GA)检测试剂盒is launched between biotin labeled Gibberellic Acid (GA) and unlabeled Gibberellic Acid (GA) (Standards or samples) with the pre-coated antibody specific to Gibberellic Acid (GA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gibberellic 赤霉素(GA)检测试剂盒Acid (GA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gibberellic Acid (GA) in the sample.