粒细胞集落刺激因子(GCSF)重组蛋白

上海信裕生物科技有限公司

2015/12/16 11:55

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粒细胞集落刺激因子(GCSF)重组蛋白

Recombinant Colony Stimulating Factor 3, Granulocyte (GCSF)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species Homo sapiens (Human)

Product No. xy042Hu01

Source Prokaryotic expression

Host E.coli

Purity > 95%

UOM 50ug

Predicted Molecular Mass 18.6kDa

Concentration n/a

Applications SDS-PAGE; WB; ELISA; IP.

Endotoxin Level <1.0EU per 1μg (determined by the LAL method)

Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

粒细胞集落刺激因子(GCSF)重组蛋白SEQUENCES

The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-CLEQ VRKIQGDGAA LQEKLVSECA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQGLLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAMPAFASAFQRRAGGVLVASHL QSFLEVSYRV LRHLA

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

粒细胞集落刺激因子(GCSF)重组蛋白STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
粒细胞集落刺激因子(GCSF)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.

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