白介素5(IL5)重组蛋白
Recombinant Interleukin 5 (IL5)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Canis familiaris; Canine (Dog)
Product No. xyA078Ca01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 17.0kDa
Concentration n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1μg (determined by the LAL method)
Residues Phe20~Ser134 with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
白介素5(IL5)重组蛋白SEQUENCES
The sequence of the target protein is listed below.
F AVENPMNRLV AETLTLLSTH RTWLIGDGNL MIPTPENKNH QLCIKEVFQG IDTLKNQTAH GEAVDKLFQN LSLIKEHIER QKKRCAGERW RVTKFLDYLQ VFLGVINTEW TPES
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
白介素5(IL5)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
白介素5(IL5)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
实战分享|小鼠淋巴细胞的提取和分选之经验小结
实战分享 | 原生细胞的合成之经验分享
实验常用人肝癌细胞株(HepG2/Hep3B,HuH-7,MHCC97H,PLC/PRF/5)怎么选
原代细胞的培养方法及注意事项
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